Innovative Solutions

FISH in Tissue Sections

As a member of the Metafer product family the new TissueFISH module is designed to serve the special requirements for cell analysis in tissue sections. Based on the powerful cell signal analysis system MetaCyte, the software provides a convenient working environment to generate efficient analysis workflows.

Once the images were acquired with MetaCyte, they can be displayed in the new module's main screen. Powerful algorithms separate many cells already automatically. With the help of the mouse, or even an electronic pen device (respective hardware is required), additional cells can be easily marked for analysis. All cells marked are immediately analyzed with the comprehensive possibilities of MetaCyte. User-defined parameter sets ('classifiers') determine the cell and signal features that are obtained. Together with the analysis results a gallery image of the marked cells is generated and also displayed, allowing for immediate control of the results. Histograms summarize the data for all cells for better overview.

Cell statuses are highlighted in the overview image. Unwanted cells can be easily uncased, and cell outlines can also be corrected interactively (e.g. by connecting two separate outlines). For better overview image processing can be temporarily applied to the field of view. Separately also the analysis images can be processed to enhance the appearance of objects or signals - automatically in the background and based on previously specified algorithms in the classifier.

TissueFISH becomes especially convenient if a pen driven device is used. Best results are achieved with a WACOM DTU-2231 Interactive Display with a screen resolution of 1920 x 1080 px.