Innovative Solutions

Slide Overview and Image Stitching

VSlide is the versatile virtual slide scanner from MetaSystems. Based on the renowned slide scanning platform Metafer, VSlide combines the advantages of a motorized microscope with modern, high-quality imaging automation. Therefore VSlide is not restricted to predefined magnifications or contrasting modes. Even Z-stacks are automatically acquired, providing the possibility to virtually 'focus' through the digitized sample. 

Fluorescence Stitching

VSlide is especially strong in fluorescence mode. Based on 25 years of experience in fluorescence imaging, it reliably handles complex fluorescence images and even images with a combination of fluorescence and bright field. 

Imaging with VSlide is very easy. The system automatically reads and interprets bar code labels, and pre-scans facilitate tissue detection in bright field, transmitted light, dark field, or any other contrasting method. The final high resolution scan can be done with any microscope magnification. 

A virtual slide image is generated automatically by the dedicated stitching software. Different contrasting methods and color modes are available to be combined in one file, or in separate data sets. Since the source files are kept on the system, image stitching can anytime be repeated, e.g. to create another image with different color channel settings.

Remote Image  Viewer

VSlide users can choose from a variety of output file formats, including the revolutionary VSViewer format that comes with its own viewer source. VSViewer images can be loaded into any internet browser without any extra software, and can be inspected through the internet.

Unlimited Flexibility

VSlide supports the MetaSystems CoolCube 1c color camera (preferred for bright field imaging) and the MetaSystems CoolCube 1m monochrome camera (for bright field and fluorescence imaging). A computer controlled RGB-LED illumination provides a homogenous field, control of color/white balance, and instantaneous color switching.        

Parameter Sets and Linking

An unlimited number of VSlide parameter sets can be stored and used to automate the scanning workflow. Information on the slide, the preferred scanning parameters, and the output format can be coded into a bar code. Alternatively a processing file, created e.g. by an external database, can control the workflow. Different scans are easily linked and performed subsequently without interaction. This feature facilitates the use of pre-scans for tissue detection, followed by a high magnification analysis scan. Also conditional scans based on analysis results are possible. Intelligent tissue mapping avoids scanning of empty regions („holes") within tissue.

Z-Stacks

VSlide supports Z-stack image acquisition to generate on-screen focusable virtual slides. The analysis software MetaViewer (included) offers the possibility to navigate through the focus planes of a virtual slide, thus providing a virtual microscope on screen.

Smart Slide Digitization

Once a virtual slide is created, all the spatial information of the originally acquired image tiles remains accessible. This allows for precise relocation of any part of the image under the microscope, or even for subsequent acquisition of selected regions using different parameters.

Tissue mapping functionality offers the possibility to create an unlimited number of smart workflows. Regions of interest (e.g., tumor regions in a tissue section) can be easily marked in the VSViewer environment. Data on the selected regions is then transferred back to Metafer to generate a target list for additional scans. Once the target list is available, the selected regions on the slide can be re-scanned with other magnifications, contrasting methods, or scanning parameters. Since VSViewer is internet ready, the selection of regions can even be done remotely. Provider of slide digitization services can use this feature to allow their customers for selecting their preferred regions from their desktops.

In combination with a laser micro dissection system (e.g., PALM MicroBeam of Carl Zeiss) regions of interest can be marked in the virtual slide, and the region specifications can then be transferred to the dissection system.

Imaging Consecutive Sections

Often it may be useful to scan subsequent sections of the same tissue block, labeled with different staining methods (e.g., H&E and fluorescence). Up to now it was, however, not possible to automatically find the corresponding regions on both slides. With the VSlide tissue matching functionality users can easily match both sections in the VSViewer. Once matching is done, any region selected in the first slide can be re-located in the second slide.

Automated Image Enhancement

With the new image enhancement possibilities of VSlide any raw image can be automatically processed while VSlide is stitching the image. Like in Metafer itself, the user can test a sequence of image processing algorithms at an original image from the source data set. Once the optimal sequence is found it can be implemented to the parameter set, and it will then be applied to each image in the set. Of course the processing is only applied to the result image, and the raw data remains untouched. Images can therefore be created based on different processing sequenced, e.g. to accentuate different features of the acquired specimen.

 VSViewer Images

The following images can be directly loaded into the VSViewer. With the VSViewer any VSlide image can be reviewed in an internet browser; no extra hardware is required. VSlide automatically generates PHP scripts to provide a gallery of images, and also the viewer itself is embedded in a PHP script. The viewer includes the following functionality:

• Zooming and panning (with the mouse or using the toolbar on the left)
• Adjusting the image to a pre-defined magnification using a drop-down list
• Navigating through the image with the navigator preview
• Adding annotations and defining regions of interest
• Adding notes
• Quick navigation to previously defined annotations and/or regions of interest
• Capturing screenshots and saving them locally into JPG files
• Visualization of original camera fields (fiels of view / FOV). This function is only available if in the VSlide parameter set the respective switch was set

Images Links

	Gallery This link directs to the main gallery of VSlide demo images on this site. Use the gallery icons to navigate to the subfolder(s) of interest, and to receive further information about the images. A click onto the image icon opens the image in the VSViewer. For details on the images please refer to the image descriptions below.
	Human Cerebellum Image of human cerebellum, acquired with Metafer VSlide using a 10x objective. The image has been captured with a b/w CoolCube CCD camera and an electronic LED illumination device. This image does not contain FOV information.
	Guppy Gills Two images from the same specimen, both acquired with Metafer VSlide using a 63x objective, a b/w CoolCube CCD camera and an electronic LED illumination device. The first image was stitched in true color mode, the second image in mixed grayscale mode. Both images contain the information of all three color channels (RGB). The images show the gills of the common guppy (Poecilia reticulata).
	Mouse Two images taken from a sagittal section of juvenile mouse (whole animal). The first image shows the complete specimen acquired using a 10x objective. In the head region a polygonal region of interest is marked. This region acquired again using a 40x objective. The result of this second scan can be seen in the second image. Both images were captured with Metafer using a b/w CoolCube CCD camera and an electronic LED illumination device.
	Pancreas Fluorescent images of a rodent pancreas section. Signal channels label cells within the islets of Langerhans (green: insulin; red: glucagon). Counterstain is DAPI (blue channel). Images were acquired with Metafer using a 10x objective after a pre-scan for tissue detection with a 2.5x objective. Five copies of the same image are shown: Full color image Full color image with the DAPI channel shown in grayscale mode One image each showing a color separation of each color channel (DAPI, Red, Green)
	TMA Image of a partial tissue microarray (TMA), acquired with Metafer VSlide using a 10x objective, a b/w CoolCube 1m camera, and an electronic LED illumination device. Single images of each TMA core can be accessed by click into the respective region of the overview image.